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Further assay details were outlined by da Rocha et al. [ 26].
Further assay details including primer/probe sequences, PCR conditions and endogenous references have been published previously (Schache et al, 2011).
Further assay optimization can be accomplished by conducting proficiency panels that specifically focus on the incorporation of identified crucial assay variables.
Then the animals were anaesthetized, and blood and liver samples were also collected for further assay.
The animals were anaesthetized, the blood and liver samples were also collected for further assay.
This result implies a need to further assay construction equipment operation to critically isolate influences on operator skill development.
Therefore compound 23 deserves further assay to determine its anti-inflammatory properties, since it lacks photoreaction properties and mutagenicity-related side effects.
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The collected serum was stored at –20°C for further assays.
The collected sera were stored at −20°C for further assays.
The RT-PCR-negative samples were further assayed by Northern-blot hybridization.
Thus, the temperature of 30 °C was chosen for the further assays, because this temperature is close to the room temperature.
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