Changes in the functionality of epigenetic machinery in addition to exposure of the genome to environmental factors might therefore also contribute to increasing epigenetic diversity with age (Fraga & Esteller, 2007).
Functionality of immunosuppression-implicated machinery may be also modulated by differential nutritional and bioenergetic MDSC behaviors.
Functionality of the TLR-based sensing machinery in human dendritic cells is definitely susceptible to age-related changes.
DOI: http://dx.doi.org/10.7554/eLife.04885.016 We further tested the functionality of calcium-handling machinery in myobundles by biochemical stimulation with caffeine and acetylcholine (ACh).
Therefore, to test the functionality of the death machinery, cytochrome c was added to S-100 extracts from p65 null or reconstituted cells and caspase activation was assessed.
The cell extract is prepared in a way that preserves functionality of the protein synthesis machinery.
They suggested that the development of chemoresistance towards anti-neoplastic drugs in melanoma cell lines could be the results of a changed functionality of the general translation machinery.
Likewise, the basal splicing factor U2AF65 can influence the functionality of the 3′end processing machinery by assembling at different polyadenylation-associated RNA elements, including the USE.
Interestingly, confocal laser microscopy and lentivirus transduction indicated expression and functionality of the viral fusion machinery, consisting of the viral fusion (F) and attachment (H) glycoproteins, at the cell surface.
The situation is further complicated by the fact that due to the partial functionality of the error correction machinery in condensin mutants, condensin is unlikely to be essential for sister kinetochore biorientation, but rather provide a bias that facilitates sister kinetochore capture and efficient error correction.
Secondly, in trans expression of exsA in the transporter mutants restored the ExsA-dependent functincludinguding expression of T3SS genes and production of effector proteins (Figure 3A, 3B), which precludes the possibility that null mutation of the transporter may also affect the functionality of the type III secretory machinery.
