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Fresh serum free DMEM-F12 media was added to the cells.
Fresh serum samples were analyzed.
Fresh serum samples were drawn after an overnight fast.
Fresh serum samples were collected daily in 10 ml tubes and used immediately.
Fresh serum was collected and cooled after sampling, and then stored at −20°C until analysis.
Fresh serum was obtained from all of the patients and normal subjects for the present study.
Similar(31)
Cells were incubated with transfection compound for 8 hr, then washed with fresh serum-free media before immediate drug treatment with TGFβ and ITF2357.
Then the cells were switched to fresh serum-free medium and incubated for 14 h.
Cells given fresh serum-free medium were used as controls.
The cells were fed with fresh serum-free medium and cultured for an additional 14 h.
SB were then re-cultured with fresh serum-free medium and supplements in low adherent dishes.
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