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From the same animals, fresh sera were also prepared.
Briefly, fresh sera were diluted in gelatin-veronal-EGTA buffer (GVB) in microwell plates and washed RBCs were added.
Both culture supernatants and fresh sera were analyzed by ELISA for IL-17A levels.
Tumor markers were measured in fresh sera using Architect I2000SR CMIA technology (Abbott Diagnostics, Abbott Park, IL).
Lipids were measured at the Department of Clinical Chemistry, University Hospital of North Norway using fresh sera from the first visit (Furberg et al., 2005).
Such false-positive reactions can also occur when testing "fresh" sera because of a phenomenon that occurs in samples that are not frozen and heat-inactivated, as was suggested for the BoHV1 gE ELISAs [ 16].
Similar(52)
Immunoglobulins were added to cultures at the concentrations indicated in the text either in the presence or absence of 1% fresh rat sera as a source of complement.
The low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and lipoprotein (a) (Lp [a]) were determined after separating the lipoprotein fractions from fresh fasting sera by sequential ultracentrifugation.
This was observed when HIV-1 was opsonized not only with fresh human sera but also when HIV-1 was opsonized in seminal fluid, despite the tenfold lower amount of C3 fragments in semen 33.
After 4 days in vitro (DIV) 400 μl of the medium was replaced by fresh medium and either sera or the GnRH analogue was added (see below).
RT-PCR (11) was performed as a rapid molecular tool to detect and type DENV only in acute-phase sera and fresh tissues from patients who died, hospitalized patients, and outpatients whose disease severity was characterized by thrombocytopenia, hemorrhagic manifestations, or both (n = 282).
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