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The larvae were transferred to fresh leaflets 4 days later.
Total genomic DNA was isolated from fresh leaflets harvested on one-month-old plants using Nucleospin 96 Plant II (Macherey Nagel, Germany) according to manufacturer's instructions.
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The model is also used to examine differences in the mechanics and fluid dynamics yielded by fresh valve leaflets and glutaraldehyde-fixed leaflets similar to those used in bioprosthetic heart valves.
The surviving larvae were carefully moved to a new petri dish with fresh potato leaflets and reared to adulthood at 23°C.
A guy hands me a fresh stack of leaflets.
The campaign offices - and yes, there are several - bustle with energy as people come in and out to pick up fresh instructions and leaflets.
The time spent going through the malaria leaflet and showing mosquitoes (leaflet or fresh samples) was around 15 min in total, with negligible variability.
Morphometric analysis of collagen and elastin fibers revealed no significant alterations in treated leaflet tissues relative to fresh tissues.
After plastering the staff canteen with leaflets, we headed back out into the fresh air and called it a day.
For extraction of PPOs, leaflets or roots were homogenized in the threefold volume (v fresh weight−1) Sörensen buffer using micro pistils.
Officials today said around 70 sites had been targeted and leaflets had been dropped on three Shia suburbs of the city warning of fresh air strikes.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com