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Most things are jumble sale prices, and some things might even be free – plant cuttings, say.
Nurseries sell Duck Foot for $2.50 to $5.95 a plant, and the American Ivy Society gives a free plant to those who join, for $20 (www.ivy.org).org
The fresh and disease free plant material was washed twice with double distilled water, shade dried at room temperature for 21 days and then grinded to powder.
AGO, ARGONAUTE; ARF, auxin response factor; DCL, DICER-like; EST, expressed sequence tags; LCO, lipochitooligosaccharide; miRNAs, microRNAs; N-PNS, nitrogen free plant nutrient solution; pri miRNA, primary microRNA; RACE, random amplification of cDNA ends; WGS, whole genome shotgun.
Surface sterilized seeds were planted in a 4" pot filled with 1 3 (v/v) mixture of sterilized vermiculite and perlite (Hummer International, St Louis, MO) and watered with nitrogen free plant nutrient solution (N- PNS).
Pots were watered regularly with ¼ x nitrogen free plant nutrient solution (N- PNS) and maintained in a controlled environment plant growth chamber (16 h light; 25°C; 30% relative humidity).
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