For the determinations we used a test kit (nitrate cell test; Spectroquant 1.14542.0001; measuring range 0.5 to 18.0 mg/L NO3-N; Merck, Darmstadt, Germany).
For the determinations of CMT solutions, the seismograms are corrected for the seismometer response, band-pass filtered between 20 and 50 s, and integrated with respect to time to obtain displacement seismograms.
For the determinations of holocellulose and hemicellulose contents, lignin was degraded by resuspending the starch-free residue in 1.6 ml of NaClO2 (400 mg NaClO2 / 60 ml of deionized water) and 0.2 ml of 99.7% acetic acid (CH3COOH), followed by incubation of the suspension at 80 °C for 60 min.
For the determinations of cellulose, the cellulose was dissolved in 67% (v/v) H2SO4 (1.0 mL) with shaking at 25°C for 1 h, and then 10.0 μL aliquot was used for the anthrone/H2SO4 method.
For the determinations of primary root lengths, seedlings were grown on liquid medium with P. indica spores under the different Pi conditions for 14 days, stained with trypan blue for 5 min and then placed on glass slides.
For the determinations of the Km values of both UDP-GlcNAc and the mannopeptide Ac- ATPT PVTAIG-OH, an enzymatic assay was performed with a series of different substrate concentrations.
