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Cells were nucleofected with the indicated siRNAs and cultured for indicated periods of time.
hBMEC cells were treated with sublytic concentrations of suilysin protein for indicated periods after pretreatment regimens.
SH-SY5Y cells were exposed to H2O2 for 1 h and then cultured for indicated time.
Additional media was added and the infection was continued for indicated time periods [38].
For this purpose, fibroblasts expressing ectopic Nab2 were incubated with TGF-β for indicated periods.
The transfected cells (72 h post-transfection) were then treated with S100A14 for indicated periods.
Cells were infected at an MOI of 1.0 for indicated times.
Aliquots of 106 cells were stimulated with CXCL12 (Preprotech) for indicated time.
The mixtures were then incubated at 37°C for indicated time period allowing DPPIV-enzymatic reaction.
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Unless otherwise indicated, values represent means ± SEM for indicated number of rats.
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