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For EAG recordings, the head of each 4 8 day old control or dsRNA-injected female A. gambiae was excised at the occipital opening and placed on the reference glass electrode containing Hayes mosquito Ringer solution [51], mounted in a humidified air-stream (90 98% RH) and the antennae exposed to test ligands eluting from a gas chromatographic (GC) column [52].
For EAG measurements we also used the American Z- and E-strain (kindly provided by Charlie Linn; Cornell University, New York, USA).
The 12-month survival rate was 94.3% for EAG repair and 95.9% for COS repair.
The 30-day mortality rate was 1.5% for EAG repair and 3.1% for COS repair (P =.59).
The intermediate-term cumulative survival rate at 24 months was 84.9% for EAG repair and 80.3% for COS repair (P =.48).
Consequently groups B and F were equivalent (received 3TC only) except for eAg status, and likewise C and G.
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In addition, the 95% CIs of eAG for any A1C value imply uncertainty of the "true" mean and more so at very high A1C levels.
Only antennae showing a response at least double the noise level to a puff of air over 1 µg geranylacetone in a 5 ml syringe were used for recording EAG responses to the ligand mixture.
Phratora vitellinae L. (Brassy willow beetle) adults for additional EAG studies were collected in the field on Grey poplars at Göttingen University.
This pronounced hyperexcitability indicates a major role of Eag channels for maintaining baseline neuronal excitability.
We also published estimates of Eag and Eig for PM2.5 among a subset of the Seattle panel study subjects (Allen et al. 2004).
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