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For each target x of that subject (n targets).
For each target in DUD-E, all actives are fetched.
For each target company, try to find a contact that can provide an introduction.
For each target, 100 trials were presented.
For each target, forward and reverse primers and an internal probe were synthesized.
For each target peptide, the binding energy was calculated using those 99 energy matrices.
For each target, the native structure was introduced in the scoring ensemble.
For each target gene, absolute copy number was divided by the NF.
For each target, the scoring ensemble was evaluated using GA, CF and CF-GA methods.
For each target gene, probes were selected spanning a wide range of design parameters.
For each target gene, primers were selected using the Beacon Designer software (Bio-Rad, Hercules, CA).
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