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For each sorted fraction the DNA histogram was evaluated using FCM analysis.
For the first requirement, we computationally modified the number of random bases in each template (from 4 to 38 bases for LTs, and 4 to 24 bases for STs) and determined the number of unique barcodes for each sorted index and template (Figs 2a and S2a, gray lines).
(7) Initialize =, =, = ; for each sorted with the increasing order of do.
The contamination of sorted fractions by other chromosomes was determined for each sorted run after analyzing 1,000 chromosomes on a microscopic slide.
DOI: http://dx.doi.org/10.7554/eLife.09083.008 We quantified the frequency of IgH sequences expressing individual VH gene for each sorted B cell sample and then compared the VH gene usage between two B cell subsets.
To overcome these obstacles, we have coupled high-dimensional (Hi-D) FACS sorting with unique IgH multiplex PCR technologies, which allow inclusive amplification of IgH transcripts for each sorted B subset and ultimate sequencing of these sequences.
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For each many-sorted language with $n$ styles of variable, we may introduce a one-sorted language, which comes with a one-place predicate, $S_i$, for each sort in the many-sorted language.
Reads for each individual were sorted and trimmed by the perl scripts Split in the SEG-Map package.
For each document, a sorted list of nearest neighbors was identified as those having the highest cosine similarity scores.
In particular the mean fitness values reached for each experiment where sorted in ascending order.
The resulting RAST and KO (KEGG Orthology) assignments have been tabulated for each genome and sorted by GenBank identifiers.
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