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For determinations of relative FG-3019 explasma, plasma FG-3019 antibody concentrations were determined by a sandwich ELISA using an exon-3 (VWC homology domain) CTGF peptide for capture of FG-3019 followed by detection with goat anti-human IgG linked to alkaline phosphatase.
For determinations of ultrastructural morphology, muscle tissue sections were fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4).
For determinations of Mg2 +-independent nSMase activity the cells were lysed in 100 μl neutral lysis buffer without MgCl2.
For determinations of Mg2 +-independent nSMase activity BODIPY-SM was added to neutral reaction buffer without MgCl2.
For determinations of DNA content, total protein/DNA ratio and membrane/total protein ratio, the medium was aspirated and the culture was rinsed with a buffer consisting of 154 mM NaCl and 10 mM sodium phosphate (pH 7.4).
For determinations of DNA content and the protein/DNA ratio, the medium was aspirated and the culture was rinsed with a buffer consisting of 154 mM sodium chloride and 10 mM sodium phosphate (pH 7.4).
On day 2, pulmonary arteries were isolated for determinations.
At inclusion, 3rd and 7th day, venous blood samples were obtained for determinations above were obtained.
A novel active microband-electrode probe is introduced for determinations of trace lead in various natural samples.
Each of the 60 μL MT-I or MT-II hydrolyzates was used for determinations of the DPPH antioxidative activities by spectrophotometry.
A 'dry' mercury-capture method has been evaluated for determinations of mercury release from a pilot-scale gasifier equipped with a hot gas filter.
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