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For assay of MMP-3 activity, casein zymography and for assay of MMP-9 and -2 activities, gelatin zymography were performed as described previously [10].
For assay of released LDH, supernatants were collected.
Dotted line indicates minimal detectable threshold for assay.
The set is ideal for assay validation, and as a reference set for secondary screening.
We encourage researchers to get a sample of our cells for assay development.
The interacting mechanism was studied and the optimum conditions for assay of PY were also investigated.
Total time for assay completion was approximately 12 h.
Tabatabai, M. A. & Bremmer, J. M. Use of p-nitrophenyl phosphate for assay of soil phosphatase activity.
All other reagents and solvents for assay were of analytical reagent grade and were purchased from Sigma-Aldrich.
The visceral organs were rinsed of residual blood, blotted up, weighed and stored at −20°C for assay.
(31) ASTM E298-68, "Standard Methods for Assay of Organic Peroxides," effective September 13 , 1968 into § 571.116.
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