The use of a proofreading polymerase for the amplification and sequencing supports the latter hypothesis (data not shown).
For many of these enzymes, this is achieved by high selectivity for the correct substrate, with a proofreading step for removing incorrect nucleotides if the selection step fails (for a recent review see [ 1]).
The authors would also like to thank Eric Diehl for providing a proofreading of the manuscript.
Following RNA hydrolysis, an adapter primer was annealed to the 3' end, and the produced fragments were PCR-amplified for 22 cycles with a proofreading enzyme.
For a GCSE in proofreading, perhaps?
Mrs. Grippaldi, 31, is a proofreading manager for marketing publications at OppenheimerFunds, the mutual fund company in New York.
Because we used a proofreading polymerase for PCR amplification and a high fidelity RNA-dependent DNA polymerase for cDNA synthesis, these nucleotide substitutions can be considered authentic.
Because its genetic material is packaged in the form of RNA -- rather than the more common DNA, which converts to RNA before reproducing, allowing for an intermediate "proofreading" step -- random mutations during replication are relatively common and are passed on intact to the virus's offspring.
Sacked from Simon & Schuster for an expensive proofreading error made by an underling (an all-important "L" was left out of the last word of the title of 50,000 deluxe editions of the Nancy Drew mystery "The Secret of the Old Clock"), Rannie turns to a life of crime.
We are grateful to Brenda J. Landau for an English proofreading of this article.
By taking a reasonable fee for proofread service, you can become a go-to person for anybody willing to give their last essay a once-over without paying an arm and a leg to a professional proofreading agency.
