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Although often used confusingly in the literature, accuracy should not be confounded with trueness and precision.
The following parameters were determined: linearity, trueness, repeatability, within-laboratory reproducibility, decision limit (CCα), detection capability (CCβ), selectivity and robustness.
Due to the lack of reference materials for these analytes, trueness could not be evaluated.
A CRM can be used during method validation to assess trueness and ensure traceability of the results.
According to trueness, LOQ was determined to be 0.008%, since below this level bias showed a drastic increase.
Nevertheless these results are only an indication of the assays' trueness, and more studies should be performed to increase the reliability of these data.
The trueness of the reference method should be checked with National Institute of Standards and Technology reference materials or other traceable materials.
Alternatively, a measurement procedure may be precise but inaccurate due to lack of trueness (ie, the procedure is biased).
The measurement error can be systematic (bias) qualifying the untrueness (see "Measurement trueness") or random qualifying the imprecision (see "Measurement precision").
According to 2002/657/EC [ 1] the trueness at the selected validation levels should be between 80 and 110%.
Trueness: the trueness (expressed as lack of agreement or bias) should be within ± 25% of the accepted reference value over the whole dynamic range.
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