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Following transfection, cells were cultured for 8 h and treated with DMSO, XIE62-1004, XIE2008 or rapamycin for 16 h.
Following transfection, levels of plasmid expressed proteins should steadily increase.
Following transfection with siRNA, cells were cultured for 2 days before use.
Following transfection, the assay plates were wrapped with Parafilm and floated on a revolving water bath.
Following transfection, cells were harvested and analyzed at the indicated times.
Following transfection the cells were left to recover overnight at 37°C and 5% CO2.
Following transfection, luciferase activity was standardized to Renilla luciferase plasmid activity to normalize for transfection efficiency.
Following transfection, the siRNA successfully suppressed PTK7 mRNA and protein expression.
Following transfection of miR-124, 370 mRNAs were specifically recruited to Ago2.
Following transfection and selection, strong expression was observed.
Following transfection, cells were treated as indicated for 24 h.
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