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Following selection, five studies were eligible.
Following selection, dual-gene expression percentages in single-gene vector cotransduced and dual-gene vector transduced populations were similar.
Following selection on digoxigenin-binding activity via phage display and colony screening, several DigA variants were thus obtained.
Following selection, plants derived from single embryos were regenerated and were screened for the presence of the respective transgene by PCR using specific primers.
Following selection of individual puromycin resistant clones, genomic DNA was isolated using Trizol (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and PCR amplified targeted regions were sequenced.
Following selection, cells were FACS sorted and GFP-positive cells were obtained.
Following selection of the antigen-specific clones the immunoglobulin genes can be retrieved from the clones.
Following selection of a suitable field of view, coverslips were maintained at 31°C for 5 minutes.
Following selection and expansion of cells, clones expressing UBP43 were identified by immunoblotting with anti-FLAG antibody (M2; Sigma).
Following selection, stable clones were isolated by limiting dilution.
Following selection, each girl has periodic exposure to the chosen model via the iPad (relatedness).
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