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Following MO paw injection, the animals were transferred to a restraining cage (Bollman Cage; Natsume Seisakusho, Tokyo, Japan).
Following MO injection, the changes in paws flinching as the early onset of pain and paw withdrawal latency (PWL) in response to thermal stimulation were measured as delayed-onset hyperalgesia.
The flinching was evaluated by plotting a time course following MO injection, then calculating the sum of flinching up to 8 h after MO injection.
It was a second gold in three days for the Great Britain team, following Mo Farah's triumph in the 10,000m on Tuesday, and there in the promise of much more to come with Dai Greene, in the 400m hurdles, and Michael Rimmer, in the 800m, the fastest qualifiers from their semi-finals last night and Farah cruising through to the final of the 5,000m.
The British captain, who had earlier this week told her team to "burn the ships" in a rallying speech, had given it her all and had nothing left after securing a second gold of the championships for Britain following Mo Farah's 10,000m triumph on the opening day.
In our present zebrafish studies, however, a pancytopenia phenotype was observed in TERT-knockdown embryos following MO injections, which was rescued by following injection of a zTERT mutant (ΔTR-zTERT) lacking the TR domain, suggesting that the cytopenia is not due to direct inhibition of authentic telomerase activity itself.
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A grand novel in comic-strip form, the book follows Mo and her friends as they find lovers, break up, recombine (one even marries a man) and raise children over more than two decades.
Following this, Mo has been deposited by DC-sputtering onto the SiO2.
Percentages for the various phenotypic groups obtained following MO-tsl injection were determined and are representative of 3 independent experiments; each experiment was performed with at least 70 injected embryos.
Morpholinos were as follows: Nhsl1b: (MO E4I4 5′-CTAAAAGTTTAACTTCTCACCCGTG-3′; MO exon1 ATG 5′-CGGGAAACGGCATTTTAAATCCTGT-3′), 5 ng; Hoxb1a: (Cooper et al., 2003), 2 ng; Pk1b: MO1 + MO2 (Rohrschneider et al., 2007), 2 ng each; Scrib MO: (Wada et al., 2005), 5 ng; Vangl2 MO (Park et al., 2008), 3 ng.
The sequences were as follows: Control MO: 5'-CCTCTTACCTCAGTTACAATTTATA-3' [50], tbx3b MO: 5'-TGGATCTCTCATCGGGAAGTCCAG-3', tbx2a MO: 5'-ATCGGTGCATCCAAAAAGCCAGAT-3'.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com