Following measurement of small, short-lived traces of benzene in one monitoring well and a subsequent high reading supplied as the result of a laboratory mix-up of samples, the project was issued a shutdown notice from the Queensland Government.
Following measurement of AHR, the lung of each rat was lavaged in situ with 3 successive 1 ml volumes of saline instilled by syringe.
Following measurement of chromatic aberration (Additional file 3, part A) the aberration was corrected by scaling of the BPI image (Additional file 3, part B).
Following measurement, the middle leaflet was severed at the petiole and scanned on a flatbed scanner (HP Scanjet 3670; Hewlett-Packard Australia Pty Ltd ,Blackburn, VIC, Australia).
Following measurement, the samples were immediately placed in Eppendorf tubes containing 20 μl PBS (phosphate buffered saline) and frozen at − 20 °C.
Following measurement, data sampled at 30 Hz will be exported using the manufactures software, Data logger reader (T&T medilogic Medizintechnik GmbH, Berlin, Germany).
Following measurement of protein concentration using the BCA (bicinchoninic acid) protein assay kit (Pierce), the homogenate was aliquoted to obtain a protein amount of 50 μg/tube.
Following measurement of the fluorescence signal in the relevant gated region, 5 × 10 side population cells were sorted into Eppendorf tubes on ice and centrifuged, before total RNA was isolated for subsequent mRNA expression analysis, as described below.
Following measurement of internode lengths, the internodes (excluding the nodes) from the apical bud to the base of the shoot were excised into liquid nitrogen in the field and stored at -80°C.
Following measurement of each analyte, the ratio of the multiplicity of median (MoM) sLHCGR :MoM hCG-sLHCGR was calculated for each serum.To visualize the difference in the distribution of results in DS cases and controls, the data were log-transformed and plotted in a box-plot (see Figure 1a).
The following measurement instruments and measures were administered: child questionnaire, parent questionnaire, accelerometers, anthropometric measures, school management questionnaire, interview with those responsible for the cafeteria/food retail and the vending machine, and a school environment audit instrument.
