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Following clarification by ultracentrifugation (100,000×g for 45 min), the cell lysate was applied on HisTrap chelating nickel affinity column (GE Healthcare).
Following clarification and discussion, these were then subjected to independent ranking.
Following clarification, the homogenates were centrifuged at 100,000 × g for 1 h at 4°C.
Following clarification by centrifugation the supernatant was diluted fourfold with MQ water and dialysed thrice to remove urea and enable Nedd8 refolding.
Following clarification, the supernatant was filtered using a 0.22 µm PES filtration unit and transferred to a vessel for buffer exchange.
Following clarification by centrifugation at 18,000 g for 30 min, samples were aliquoted into fractions and either frozen in liquid nitrogen and stored at −80°C or used immediately.
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The soaring number of passengers filing claims follows clarification of European Union rules in October 2012.
The design procedure utilized in this paper follows "clarification", "analysis", "design", "simulation", and "evaluation".
All the questions which needed reiteration of the content that has been explicitly been taught in the seed or in any other previous lecture in the course are categorized to follow clarification strategy.
Following this clarification, TSS antero-lateral and TSS anterior approaches can be classified as "intermuscular approaches", while the direct lateral approach, with a standard or mini-incision, is classified as a "transmuscular approach".
Following any clarification, participants signed consent forms.
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