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In order to evaluate the capacity of insulators to block insertional mutagenesis, we first inserted cassette 234-β-EGFP on chromosome 4 at site RL5 to determine if insertion of an expression cassette would activate the flanking genes.
As starting position of an indel we took the position of the first inserted nucleotide for an insertions and the position of the first changed nucleotide for a deletion.
Insertions are described by a '+' or a '-' after the nucleotide flanking the insertion site, followed by the position of the first inserted nucleotide of the intron (beginning of intron = +1, for insertions flanking the acceptor site: end of preceding intron = -1), followed by 'ins' and the length of the inserted sequence.
Their implant was first inserted in 1970 and is still used today, with some minor alterations.
The fragment of DNA to be amplified is first inserted into a cloning vector.
For low implantation doses, Mo first inserted into austenite cells inducing austenite lattice expansion.
It was in a boxing ring, of course, that Ali first inserted himself into the consciousness of the British people.
To facilitate generation of the ins:GCaMP6s; cryaa RFP construct, MCS (MCS2) were first inserted downstream of the insulin promoter to yield ins:MCS2; cryaa RFP.
During IMD processing, the film having the same shape as mold cavity is first inserted into the mold then molten polymer is injected into the cavity.
The Brahmanas of the Yajurveda were at first inserted at various points in the texts alongside the material on which they commented.
The researchers, who describe the technique in Nature, first inserted a relatively large hypodermic needle into the forearm, inserted the endoscope through the needle tube and then removed the needle.
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