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Methanol extract (ME), obtained by successive solvent extraction, its most effective liquid-liquid n-butanol fraction (NBF) and the flash column chromatographic sub-fraction (SFI), were evaluated for in vitro α-glucosidase (yeast) and α-amylase (porcine) activity inhibition.
The extraction process yielded 4.08% of the methanol extract (ME), 0.42% of n-hexane fraction (HF) and 3.56% of methanol fraction (MF) (Table 1).
Strains were grown in a base medium composed of 100 g/L malt extract (ME) (Sigma-Aldrich, St . Louis MO, USA).
Then, the methanol extract (ME) was centrifuged at 5000g for 15 min at 35 °C.
Subsequently the organic phases were combined and evaporated to yield 221 mg of oily mycelial crude extract (ME) in total.
The highest level of TPC was detected in the methanol extract (ME), whereas the lowest was observed in the chloroform extract (CE).
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PEE-Petroleum ether extract, CE-Chloroform extract, ME-Methanol extract.
PEE-Petroleum ether extract, CE- Chloroform extract, ME-Methanol extract, AE-Aqueous extract, BHT-Butylated hydroxytolune.
PEE-Petroleum ether extract, CE- Chloroform extract, ME-Methanol extract, AE-Aqueous extract, AA-Ascorbic acid, GA-Galic acid.
PEE-Petroleum ether extract, CE-Chloroform extract, ME-Methanol extract, AE-Aqueous extract, AA-Ascorbic acid, BHT-Butylated hydroxytolune.
PEE-Petroleum ether extract, CE-Chloroform extract, ME-Methanol extract, AE-Aqueous extract, AA-Ascorbic acid, BHT-Butylated hydroxytolune Fig. 2 IC50 of different extracts of C. pallida stem and standard antioxidants.
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