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In contrast, TB40/E, which has not been extensively propagated on fibroblasts, harbours fewer mutations, and retains the ability to replicate in a wider variety of cultured cell types.
Ectopic expression of telomerase in early passage cells prevented telomere dysfunction and activation of the p53-p21 pathway [ 15], and readily yielded immortalized clones, some of which have been extensively propagated [ 22].
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Collapse of the slabs always occurred through a combination of the crushing of the concrete after flexural cracks propagated extensively, and punching shear.
Other local factors may dictate how extensively modifications are propagated on individual genes.
Unlike primary cells, stem cells can be propagated extensively in vitro and because they can be derived from a clonal source, their progeny have a homogeneous genetic profile.
The region of interest (chr11 46724721-46724734) has a high number of mutations in MCF7 compared with the reference genome, a common observation for cancer cell lines and cell lines that have been propagated extensively.
Explanations for this may be that the UOK262 cells are from a distant metastasis and have been propagated extensively in vitro, or that the patient may have received multiple rounds of therapy that have impacted the metabolism of these cells.
Thus, when brain-derived 22L prions ('brain[22L] prions') were propagated extensively in neuroblastoma-derived PK1 cells, the CPA characteristics of the resulting prions ('PK1[22L] prions') changed, but were restored when the prions were again propagated in the brain (Li et al, 2010).
Then we extensively investigate the numerically propagated orbits for 200 and 1000 years, applying the results from these simplified models and seeking proper explanations for the underlying dynamics.
MDR-TB epidemics propagated more extensively after TB prevalence had fallen.
As the judge in Ibrahim's case noted, "once derogatory information is posted to the [Terrorism Screening Database], it can propagate extensively through the government's interlocking complex of databases, like a bad credit report that will never go away".
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