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Additionally, the in vivo experimental time span and presence of host defenses differ from chronic infections and the chemical microenvironment of both in vivo and in vitro biofilms is seldom taken into account.
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Among wet laboratory approaches to study the ecogenomics of organisms, next-generation sequencing is considered to be one of the most advanced technologies to reveal the genome of organisms with a short time span under any experimental condition including under eco-stressors.
After electroporation using the combined HV/LV pulse strategy, no vesicle formation was observed and the transfected specific siRNA significantly suppressed model gene expression for up to 72 h (Figs 5 and 6); a time span suitable for experimental approaches that include specific knockdown.
The number of synaptic current events occurring in this time span depended on experimental conditions, but was generally higher than 500 and usually around 1500.
Therefore, a more thorough analysis with a new experimental design might be necessary to establish the time span after which convection in such complex system becomes stable.
Accounting for the magnitude of a 'bulge' in the 24 72 h time span (in the plot of experimental data minus best-fit function) would bring the swelling estimates up to ~3.2 % for the lumps (Table 4).
The decision to use experimental data to protect public health is important because the time span of widespread APM use is still too brief to have produced solid epidemiologic data.
They also tend to be more experimental, beholden to a smaller budget and produced in a shorter time span.
To our knowledge, there are hardly any experimental tests on the stability of socially learned behaviours over a time span longer than a few months.
The lower tissue concentrations compared to other studies [ 16] is to be seen in context with the short time span between final drug application and euthanasia of the experimental animals.
The ten sessions of core treatment for the third experimental group receiving cooperative learning strategies also took place in the five-week time span each week embracing two sessions of 90 min.
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