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Experimental cells were then selected among those that were capped within 6 hours and subsequently used for infestations20.
Experimental cells were cultured with daily 20-minute exposures to LIPUS.
Experimental cells were pretreated 30 min prior with either I3M or DMSO alone.
Experimental cells were fed the same medium as control cells except that 1 μM clorgyline was added.
The experimental cells used indium tin oxide, which is limited by the availability of indium.
The control cells also grown for the same length of time (5 days) as the experimental cells.
The comb was then replaced into its original colony and the experimental cells were checked every 12 hours during the next 3 days.
We thus opened the experimental cells one day before the expected emergence date and reported the developmental stage of the opened brood as well as its survival status.
Four built experimental cells were used to validate the numerical results.
The experimental cells was focal positive for S-100 immunohistochemical assay.
We then placed the comb into an incubator at developing brood conditions, i.e. 34.5 °C and 70% RH, and checked the experimental cells every 12 hours during the next three days.
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