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The OTUs of assA, mcrA functional genes were translated using ExPASY translation tool (http://web.expasy.org/translate/).org/translate/
The nucleotide sequence for GmRLK18-1, the RLK encoded within the rhg1 locus, was translated to its polypeptide sequence in-silico using the EXPASY translation tool (http://www.expasy.ch/tools/dna.html).html
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In silico translation (Expasy, translate tool [ 46]) and gene structure prediction (GENSCAN Web Server http://genes.mit.edu/GENSCAN.html, [ 47]) were performed on the opsin-like sequences retrieved from the draft genome of A. filiformis.
Using the ExPASy Translate Tool (Swiss Institute of Bioinformatics), all six possible translation initiation positions (3 each on 5′ and 3′ strands) were assessed to be devoid of protein coding capacity, yielding evidence that these are indeed small non-coding RNAs expressed during rickettsial infection of host endothelium.
Open reading frames were located with ORF finder (http://www.ncbi.nlm.nih.gov/gorf/), and translation was carried out using the ExPASy translate tool (http://web.expasy.org/translate/).org/translate/
Open reading frames and translation of the contigs were carried out with the ExPASy translate tool [ 10] and confirmed by BLASTx with the GenBank NR database comparison to related sequences, as well as by comparison among the T. aestivum caleosin sequences as the data set was developed.
DNA sequences were analysed by ExPASy protein translation (SIB Bioinformatics Resource Portal) followed by Protein Basic Local Alignment Search Tool (BLASTP, NCBI) analysis.
For the putative PCWDEs, the transcript sequences from the phasmids were converted to amino acid sequences using the ExPASy online translation tool [ 51].
The encoded protein sequences were deduced using the translation program at ExPASy (http://web.expasy.org/translate/).org/translate/
The amino acid sequence were deduced from the corresponding cDNA sequences using the translation tool at the ExPASy Proteomics website (http://expasy.org/tools/dna.html).html
Amino acid sequences were deduced from the corresponding cDNA sequences using the translation tool at the ExPASy Proteomics website (http://expasy.org/tools/dna.html).html
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