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Calculated masses were obtained using the ExPasy ProtParam tool (http://web.expasy.org/protparam/).org/protparam/
After purification the protein concentration was determined by UV absorption (NanoDrop), using the molecular weight and extinction coefficient calculated by the Expasy Protparam tool (web.expasy.org/protparam/).org/protparam/
Protein concentrations were calculated from the measured absorbance of samples at 280 nm using extinction coefficients from the output of ExPASy Protparam tool (http://expasy.org/tools/protparam.html).html
Extinction coefficients were calculated using the ExPASy ProtParam tool [ 68].
Firstly, ExPASy ProtParam tool was employed to determine the physical and chemical parameters of EYS.
The molecular weights and isoelectric points of the MaSODs were calculated using the ExPASy ProtParam tool [ 30].
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Molecular weight and pI was calculated using ExPASy-ProtParam tool (http://web.expasy.org/protparam/).org/protparam/
The instability index, as computed by ExPASy-ProtParam tool, was 36.33 that classified the protein as a stable protein.
The extinction coefficient was determined with ExPASy's ProtParam tool (http://us.expasy.org/tools/protparam.html).html
Enzyme concentrations were determined by absorbance at 280 nm, using extinction coefficients and molecular weights calculated by ExPASy's ProtParam tool (http://web.expasy.org/protparam/).org/protparam/
The helicase core of Mss116 does not contain tryptophan residues and its calculated extinction coefficient is small (ε280 = 18,255 M−1 cm−1; ExPASy Proteomics Server ProtParam tool [ Wilkins et al., 1999]).
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