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Expasy ProtParam classified this chimeric protein as a stable protein.
Calculated masses were obtained using the ExPasy ProtParam tool (http://web.expasy.org/protparam/).org/protparam/
EnStef parameters were predicted using the ExPASy ProtParam online tool53 and SignalP 4.154.
After purification the protein concentration was determined by UV absorption (NanoDrop), using the molecular weight and extinction coefficient calculated by the Expasy Protparam tool (web.expasy.org/protparam/).org/protparam/
Expasy ProtParam classified this chimeric protein as a stable protein and also 89.8% residues of constructed vaccine were located in favored regions of the Ramachandran plot.
Physicochemical properties were predicted using a bioinformatics tool (ProtParam in Expasy ProtParam) (Chaparro and Da Silva Junior 2016).
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Molecular weight and pI was calculated using ExPASy-ProtParam tool (http://web.expasy.org/protparam/).org/protparam/
For putative proteins ExPASy-Prosite [ 13], ExPASy-ProtParam [ 14], pSORT [ 15], and SMART [ 16], software analyses were performed.
The instability index, as computed by ExPASy-ProtParam tool, was 36.33 that classified the protein as a stable protein.
The Expasy software Protparam [25] was used to achieve this.
The extinction coefficient was determined with ExPASy's ProtParam tool (http://us.expasy.org/tools/protparam.html).html
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