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The methodology used to built the FcB1-schizont-EST library, i.e. suppressive subtractive hybridization [ 35], which was selected to ensure stage specificity, has some technical disadvantages.
At 12 hours after onset of egg production, to ensure stage synchronisation across all samples, all replicate plates from five concentrations that spanned the exposure range used in the life-history study (Cd: 0, 1.12.252.25, 4.5, 9 mg/L; FA: 0, 0.25 0.55, 0.5, 1 mg/L; AZ 0, 5, 25, 75, 150 mg/L), were washed in M9 and worms collected by gravitational isolation.
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Lloyd Richards, with his multiple directorial positions, was able to ensure staged readings at the O'Neill Center for Mr. Wilson's first three plays, followed by full productions at the Yale Repertory.
Among them, many encode RNA-binding proteins (RBPs) [4], arguing that regulated mRNA storage, stabilization and translation are needed to ensure stage-specific protein synthesis.
DOI: http://dx.doi.org/10.7554/eLife.04265.034 Examination of adult-specific alae is a sensitive physiological readout that should overcome any limitations of monitoring delays in the down-regulation of LIN-28 expression levels since scoring adult alae ensures stage-matching and accounts for any perdurance.
Zaldostanov, better known by his biker name "the Surgeon", said he would work to ensure the stage remained free of unwanted western influences.
Worked closely with management to avoid or ensure earliest stage resolution of potential or pending claims in the best interest of the corporation.
Quality control procedures ensure that stage of change and social cognitive constructs are consistently represented in all intervention materials.
Tumors were allowed to grow for 16 18 weeks prior to cell collection to ensure late stage carcinomas and increased metastasis.
Quality control procedures will ensure that stage of change and social cognitive constructs are consistently represented in all intervention materials.
Once the trees are established and productive, fruit is sampled over several weeks to ensure the stage of optimum maturity is covered.
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