Moreover, we validated both limiting states by (i) engineering a disulfide bridge in the interface of the CL state (Fig. 5) and (ii) enhancing the salt-bridge interactions that stabilize the OL state.
Moreover, the stability of T. reesei endo-1,4-β-xylanases II (XynII) was increased by engineering a disulfide bridge at its N-terminal region.
The stability of Bacillus circulans 251 CGTase was raised by engineering a salt bridge on the surface of the B-domain (Leemhuis et al. 2004a).
Engineering reports suggest a bridge carrying 60m cars a year between the island of Montreal and suburbs on the south shore is in danger of collapsing.
A joint effort with Trinity College of Arts & Sciences, the facility will create a "collision space" where faculty and students from engineering, physics and the sciences work together to develop solutions to social challenges — much as the Fitzpatrick Center for Interdisciplinary Medicine, Engineering & Applied Sciences CIEMASS) was designed as a bridge between engineering and medicine.
Each comprises a core engineering project such as a bridge over a busy road, railway or river, which together with the development or improvement of feeder routes was intended to make it easier for pedestrians and cyclists to reach destinations in their local area (http://www.lotterygoodcauses.org.uk/project/sustrans-connect2).
It is worth mentioning here that in the case of OMSVP3, engineering an additional disulfide bridge increased the specificity for only one protease, α-chymotrypsin, and decreased the specificity for other proteases [23].
"We would not do that in health or standards in engineering about how to build a bridge," said Kenneth Dodge, a professor of public policy at Duke University.
Besides, the utility of the algorithm for practical structural engineering applications is demonstrated using a bridge design example.
Next, the methods are applied to a collection of civil engineering reliability problems, which includes a bridge crane, a retaining wall and a composite breakwater.
Between the demand and supply sides, several technologies of solar thermal engineering can be applied as a bridge for an energy efficient design.
