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Early approaches viewed motivation as largely or entirely instinctive.
Early approaches in this field focused on homogeneous surfaces comprised of single type functionality.
Early approaches, such as brain lesion studies, focused on specific regions of the brain and linked those regions to intelligence and cognitive function2,3,4.
Early approaches made use of thin-layer chromatography (TLC) for purification and separation, and immunoassay and lectin-binding techniques for quantitative and qualitative analysis of specific glycan motifs19,20.
Early approaches suffered from the reversibility of integration routes and the fact that co-introduction of prokaryotic vector parts triggered uncontrolled heterochromatization. Shortcomings of this kind were overcome when Flp-Recombinase Mediated Cassette Exchange entered the field in 1994.
Early approaches using conventional antibody formats demonstrated the potential of immunotherapy, but also caused a range of undesirable side effects such meningoencephalitis, vasogenic edema or cerebral microhemorrhages in both murine and humans.
Early approaches were based on software emulation [14, 15].
Early approaches to measuring hardiness were problematic in a number of ways.
Early approaches to computer-aided design centering in an analog context date back to the early 80s [12 14].
Early approaches [55], which required a pre-alignment of biometric templates, have demonstrated the potential of this concept.
Early approaches to preserving food used variants of traditional cold boxes to solve the problem of how to take small quantities of products short distances to market.
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