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Second, there is a narrative at each CES.
Genome locations and predicted protein sequences were obtained for each CES sequence used and the results for those regions showing high (>70%) levels of identity with the human CES gene products or with opossum CES2.1 (full identity) were examined and compared with opossum and human CES sequences using the SIM-Alignment tool for Protein Sequences [ 57, 58] (see Table 1).
Similar(58)
Further, each CE is connected to a peer PE.
We first propose a discrete numerical algorithm to evaluate the instantaneous availability of each CE.
Prior to each CE, new cleaning discs were stored in high-purity (18 MΩ cm−1) water.
Each CE data point (N) was derived from a homogenization of two pooled retinas from a single animal.
We conducted model analysis to analyse the processes used in each CE model and identify the key CE components directly contributing to positive study outcomes.
Fourth, each CE collected fraction requires sufficient DNA to avoid excessive cycles of PCR, which can result in amplification artifacts such as long DNA concatemers (data not shown).
Each CE run exhibited enrichment, although the magnitude of the enrichment varied 6- to 93-fold with an average enrichment of 38.
Polarized and depolarized regions of interest (ROIs) containing mitochondrial events considered polarized or depolarized based on their red/green ratios were defined in each CE-LIF experiment.
These qPCR assays were performed using three technical replicates for each CE-coding gene and for actin A, the constitutive control; a no-template control reaction was also included.
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