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High-level expression of PD-1 (PD-1high) was detected on a significantly greater percentage of EBV-specific CD8+ T cells during AIM than during convalescence (Figure 3C).
Using flow cytometry, we observed higher frequencies of EBV-specific CD8+ T cells and higher intensity of PD-1 expression on EBV-specific CD8+ T cells during AIM than during convalescence.
Furthermore, in vivo studies of infants with RSV bronchiolitis have found blood levels of ECP to be higher during convalescence than during acute disease and therefore possibly play a role in the development of the long-standing inflammatory reaction seen in the airways after RSV bronchiolitis, and in which eosinophils play an important role [ 24, 25].
Immunoglobulin G purified from patient antisera collected during acute leptospirosis infection and during convalescence recognized epitopes in LigB7'-12 as well as in LigB9-11 (Figure 6), reflecting the expression of LigB by the infecting leptospires and perhaps its surface-available fibronectin/fibrinogen-binding domain.
The PD-1 median fluorescence index (MFI) values on A2-BMLF-1 (mean = 3.9) and A2-BRLF-1 (mean = 2.0) CD8+ T cells during AIM were significantly higher than during convalescence (mean = 2.1 for A2-BMLF-1 and 0.9 for A2-BRLF-1 CD8+ T cells); a similar trend was observed for A2-EBNA-3c CD8+ T cells.
Diagnosis is confirmed by isolation of the bacterium from body fluids or detection of its DNA by polymerase chain reaction (PCR) during the acute phase or of specific antibodies during convalescence.
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