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Direct alignment of phostensin and taperin reveals some degree of similarity along their entire length (supplementary material Fig. S7).
Direct alignment of the 35 bp Illumina reads resulted in the unique alignment of approximately one-third of the sequences.
Direct alignment of the signal peptides resulted in alignments which only had high quality (similarity level higher than 30%) in the h-regions (TMH).
Direct alignment of reads to reference alleles is also supported, but at the present time we find this modality has modest utility due to current limitations on read length.
Direct alignment of this sequence with that of the related phosphatase PTN4 [Swiss-Prot:PTN4_HUMAN] leaves a gap where the FA region would be (see Additional file 2); furthermore, probing all possible translations of the genomic sequence of PTN3 with the FA region HMM reveals no FA sequence that might be expressed in splice variants.
T-Coffee calculates pairwise alignments by combining the direct alignment of the pair with indirect alignments that aligns each sequence of the pair to a third sequence.
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The former case is particularly problematic because RNA-seq alignersometimeses prefer direct alignments to spliced alignments, causing spuriously aligned reads to accumulate on processed pseudogenes.
By comparison, direct alignments between optical maps provide a complementary view of the genetic variations between individuals, and differ from other comparative techniques in that fragment patterns, instead of nucleotide matches, form the basis of the alignments.
Splign can report both sense and anti-sense alignments for a single mRNA which may give it an advantage over the tools that report alignments in one direction, because an incorrectly directed alignment can have an identity higher than the one in the correct direction.
Complex discordances required directed alignment and assembly steps, complemented by additional curation, for their complete characterization.
The application of external fields can cause the bulk movement or orientation of a molecular species with electric field-directed alignment of liquid crystals being the most important technological application.
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