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This may indicate technical limitations of microarray analysis due to the use of nucleotide hybridization, which can introduce biases such as cross-hybridization of multiple labeled target transcripts to the same probe, increasing the rate of false positives and diminishing the quantification precision in regard to RNA-Seq.
The more stringent AFS-spec approach was able to reduce the false-positive rate of "buffalo reads" substantially to 0.07%, but only at the expense of a markedly diminished accuracy for quantification of the real meat components (Table 3).
Diminished brachial artery pulse.
Quantification of the fluorescent signal area confirmed diminished staining intensity of MT1/2 in diabetic and TETA-treated diabetic LV as compared to control.
Thus, our study provides evidences that abundance of Gigaxonin is not only univocally considerably diminished in all GAN patients reported so far, but that its quantification constitutes an essential tool to discriminate GAN from other hereditary polyneuropathies.
Densitometric quantification revealed that α-CTF levels of APP K16N were diminished by 75% and β-CTF levels were increased twofold (Fig 2F).
The quantification of the main water balance components becomes necessary to diminish the pollutants load from drainage, and its harmful effect on the environment, aggravated within a context of increasing water scarcity.
When the ethanol fraction in the substrate mixture increases beyond 48%, accurate quantification of the gluconate uptake rate can become difficult, due to the diminishing glucose uptake rate and the fixed ratio of gluconate/glucose uptake.
Quantification of phenotypic traits In order to capture the most distinct features of the phenotypes and diminish the effect of the culturing conditions, the traits were scored.
Quantification of 4N cells with phosphorylated Histone H3 revealed that mitotic U251-NG2 and U251-NG2 ctrl shRNA cells were diminished after IR compared to the U251 cells, indicating a more efficient G2 checkpoint in the NG2 positive population.
Q is for Quantification.
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