Four molecular markers developed from the sequence of the Bacterial Artificial Chromosome (BAC) clone JBnB75D10, which contains BnFLC.A10, were used for the analysis.
The majority of the SSR markers (89.4%) developed from the sequence of the foxtail millet cultivar 'Yugu1' could be effectively used in green foxtail, which is the wild ancestor of foxtail millet [ 26], implying that they share nearly identical genomes.
To fine map the QTL on the LG12 of RGT, a set of microsatellite markers (coded as UDMdSSR) was newly developed from the sequence of the apple reference genome.
The genetic marker developed from the sequence of ARF106 had the highest LOD score (logarithm of odds) for the fruit weight QTL, in both the 'Royal Gala' and 'Starkrimson' × 'Granny Smith' genetic maps (Table 2 and Figure 8).
The QTL cluster on LG10 associated with the fruit texture parameters was co-located with the Md-PG1SSR marker developed from the sequence 3 kb upstream of the Md-PG1 start codon [ 32].
Because the 1055 and 2919 tested SSR markers were developed from the sequence scaffolds of B. rapa and B. oleracea, respectively, they were thus designated as 'BrSF' and 'BoSF'.
Databases of SNPs have been developed from the sequencing of numerous accessions of cultivated rice and wild rice (Duitama et al. 2015; McCouch et al.; 2016 Xu et al. 2011).
With the exception of xa5 and xa13, the BB resistance genes are dominant in nature and the markers developed from the sequencing information of these genes are widely used in MAS (Song et al. 1995; Yoshimura et al. 1998; Gu et al. 2005; Chu et al. 2006a).
PCR was performed using SSR markers and specific primer pairs developed from the sequenced DArT markers located on the missing linkage groups (Tables S5 and S6).
To anchor BAC-based physical contigs to the genetic and cytogenetic maps, 315 sequence-tagged site genetic markers developed from the sequenced BAC clones were used [[ 4] and Jin et al., unpublished our result].
A polymorphic marker developed from the A3-2 sequence was therefore used for mapping the location of this cluster.
