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In the 19th century the term operetta came to designate stage plays with music that were generally of a farcical and satiric nature.
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David Sell is now the designated stage director.
These time points corresponded to green, véraison and ripe berries and the suspensions were designated Stage I to III respectively.
To label filamentous actin (F-actin), zebrafish embryos at designated stage were fixed in 4% paraformaldehyde in PBS overnight at 4°C, washed in PBS and manually dechorionated.
Fold change for each gene at the designated stage is indicated.
Tumor stage was determined at initial exploration, with 86 patients (27.2%) designated Stage I, 98 (31.0%) Stage II, 80 (25.3%) Stage III, 41 (13.0%) Stage IV, and 11 (3.5%) Stage V.
Because of budget concerns, seven laboratories began testing approximately 1 year before the others; these tests were designated stage 1.
Cells at the designated stage of induction of differentiation were washed twice with PBS and lysed by brief sonication.
Any metastatic disease is automatically designated Stage IV disease and with few exceptions is considered surgically unresectable.
This subgroup, designated stage 4s (s for special), has been recognized as a distinct clinical entity in all subsequent classifications of NB [ 2].
Sexual maturation was characterized as follows [ 61]: seawater immature fish were designated stage III (presence of spermatogonia and spermatocytes), whereas sexually mature males were designated stage VI (presence of only spermatozoa and few spermatogonia which is called the functional maturation stage).
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