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Design animals.
Time of day effects were observed for both drug and palatable treat, such that in the between groups design, animals showed greater intake, anticipatory activity, and post-ingestional activity in the early day.
During acquisition training of two experiments with factorial design, animals received daily access to either 32% or 4% sucrose solution, and goal tracking time was measured in one-minute bins.
When mice appeared to be in discomfort or distress as judged by independent animal care personnel with no knowledge of the protocol design, animals were euthanized.
INC participated in the study design, animals experiments, molecular biology analysis, and critically reviewed the manuscript.
As expected by task design, animals spent most of time in the central part of the maze, followed by the reward locations at the end of the arms (for a representative example, see Fig. 2A).
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BCB: study design, animal model, manuscript revision.
RK: study design, animal experiments, MRI studies.
AVS: study design, animal model, MRI studies, manuscript drafting, revision.
PPK: study design, animal experiments, MRI studies, manuscript revision.
TS: study design, animal experiments, MRI studies, data interpretation, manuscript revision.
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