However as the long form is definitively expressed and acts as an activator of hedgehog targets in response to hedgehog signalling (see below), we can conclude that midline-derived hedgehog signalling in amphioxus involves activation of ventrally-expressed target genes in ventral cells, and may also involve repression of these genes in dorsal cells, or indeed of other genes in ventral cells.
To definitively show that the luciferase/GFP expressing cells were functional stem cells, single visualized GFPhi cells were transplanted into cleared mammary fat pads.
These data definitively show that the developing male reproductive tract, although perhaps not dependent on estrogens or ER for normal development, expresses ER and is quite sensitive to the toxicological effects of exogenous estrogens when aberrantly exposed.
On the basis of these elements Zhu et al. express a certain skepticism in definitively ruling out the association between psoriasis, psoriatic arthritis, and NOD2/CARD15.
Finally, recent data have definitively demonstrated that human endothelial cells may express a previously unrecognized receptor for CXC chemokines named CXCR3B and derived from an alternative splicing of the CXCR3 gene [ 20].
As the average number of ESTs recovered per library was relatively low (few copies of ZnT sequence per library), we can not definitively conclude that tissues without ESTs for a given ZnT do not express this gene at all.
He did not remember expressing it definitively.
Here we show more definitively that Foxc1 is expressed by brain pericytes and that it is essential for normal pericyte function in the developing brain.
To definitively identify the Foxc1 expressing cell type in the brain vasculature, we used markers of ECs (PECAM) and pericyte/vSMC (PDGFrβ, smooth muscle actin-α) in conjunction with Foxc1 immunostaining.
To definitively determine whether physiologically expressed FLT3-ITD impacts the establishment of myeloid versus lymphoid leukemia development, we crossed Flt3 ITD/ITD with Runx1fl/fl (Growney et al., 2005) and Mx1-Cre mice to induce Runx1 deletion in MPPs.
However, because our previous study did not have data from pupal stages examining gene expression in each of the sexes separately, or in male and female tud progeny, we were unable to definitively identify the genes expressed in the female germline at pupal stages.
