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DNA reaction products, in particular 8-oxo-7-hydro-deoxyguanosine formed by the reaction of hydroxyl radicals (OH) with deoxyguanosine, are elevated in leukocytes and sera of patients with RA [ 44, 45].
The labelling reaction was initiated by mixing the denatured DNA, reaction buffer, unlabelled deoxynucleotide triphosphate, S-deoxycytosine triphosphate, and Kenlow fragment of DNA polymerase.
The photolithography and bulk micromachining technologies were utilized to construct the thin-film heater and DNA reaction chambers.
Figure 4 AFM images of DNA reaction of digestion by DNase I. Height scales = 8 nm except for (a).a DNA topography before digestion.
To improve these systems, we designed a 40mer duplex DNA reaction substrate consisting of recognition sequences from both U3 and U5 HIV-1 long terminal repeat (LTR) termini.
This application of a DNA reaction network to gold nanoparticle-based colorimetric detection enables highly-sensitive, field-deployable, colorimetric readout systems capable of detecting a variety of biomolecules.
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All samples and standard DNA reactions were carried out in triplicates.
The photothermal effect induced by a pulse light can be used to control DNA reactions.
To exclude the presence of genomic DNA, reactions were performed in the absence of reverse transcriptase.
Ratios of inhibitor (25TAC or 5′FAM-25TabC) to substrate (5′FAM-19TεC) were varied, while the total DNA concentration was kept constant (1 μM for undamaged DNA reactions or 500 nM for abasic DNA reactions).
As a negative control for the presence of genomic DNA, reactions were performed without reverse transcriptase (RT−).
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