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Within the ITS rDNA region, we selected the universal fungal-specific primers ITS1 and ITS4 because our preliminary studies demonstrated that these primers were more specific for fungal DNA, presenting lower levels of cross-reaction with human DNA than other primers.
Radiation causes DNA damage in tumor cells, facilitating DNA presenting in DC cells.
The functional differences between gyrase and topo IV are attributed to the non-specific binding of the C-terminal domain of the GyrA subunit that wraps DNA presenting a neighbouring T-segment for passage through the G-gate.
It contains a methyl group at the 5-position of the cytosine base, which protrudes into the major groove of the DNA presenting a potential recognition site (or obstacle) for protein binding without changing the Watson Crick base pairing.
The GIY-YIG domain has been also identified in Cho, another endonuclease involved in NER in E. coli, which is capable of making the 3' incisions only, but is able to efficiently incise damaged DNA presenting bulky lesions that are poor targets for the N-terminal domain of UvrC, and allow the C-terminal domain of UvrC to complete the repair by making the 5' cut [ 20].
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Working with DNA presented unfamiliar challenges.
Identifying DNA sequences that adopt alternative structures within the context of genomic DNA presents a major challenge.
They found wheat DNA — present some two thousand years before the grain is known to have been cultivated on the British mainland.
G-quadruplex DNA presents a potential target for the design and development of novel anticancer drugs.
At the Cold Spring Harbor meeting, there were literally hundreds of genomes or exomes (that's just the genes, which are small fraction of DNA) presented.
Although nuclear DNA is susceptible to ROS-mediated damage, mitochondrial DNA presents an even more vulnerable target.
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