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DNA PCR was performed to confirm the HPV status of tumor tissues (data not shown).
DNA PCR amplification was carried out with standard conditions using Taq DNA polymerase (Roche).
extract DNA, PCR, and prepare PCR products for sequencing.
The student will help to sort botanical specimens as well as extract DNA, PCR, and prepare PCR products for sequencing.
To calculate the PCR amplification efficiency, a ten-fold serial dilution of template DNA (PCR amplified product) was performed and followed by qPCR (Supplementary Figure 1).
For mouse tail genomic DNA, 250 ng PCR products were mixed with 250 ng wild-type (WT) genomic DNA PCR products and then denatured and annealed.
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Libraries were made using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina, San Diego, USA).
WGS libraries were constructed using the TruSeq DNA PCR-Free sample preparation kit (Illumina, Inc., CA) following the manufacturer's instructions.
TruSeq DNA PCR-Free libraries were prepared from blood and FF tissues using 1 μg of input DNA according to the manufacturer's instructions (Illumina, San Diego, CA).
TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, USA) were used to generate the sequencing libraries.
Sequencing libraries were generated using a TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, USA) following the manufacturer's recommendations, and index codes were added.
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