Sentence examples for DNA incorporating from inspiring English sources

A two-fold improvement was observed at the highest plasmid dose when using hGDNF DNA incorporating sequences found in RNA splice variant 1 compared with splice variant 2; of note, the splice variant 2 sequence is used in most preclinical studies.

After temperature cycling and treatment with DpnI to digest the parental DNA template and select for the desired DNA construct, the nicked vector DNA incorporating the mutations was transformed into E. coli.

The FISH probes (chromosome 17 alpha satellite (alphoid) DNA, or pBeloBAC 11 vector DNA) were labelled by nick translation of the DNA, incorporating either digoxigenin-11 dUTP (Roche) or biotin-16-dUTP (Roche), using a commercial kit (Nick translation system, Invitrogen).

The reaction was placed on ice for 2 minutes, 1 μl Dpn I (10 U/μl, New England Biolabs, Ipswich, Massachusetts, USA) was added, incubated at 37°C overnight to digest the parental (i.e., the non-mutated) plasmid template DNA [ 17] and the recircularized vector DNA incorporating the desired mutations was transformed into competent DH5α E. coli.

These results strongly suggest that the length of continuous DNA incorporated into B. subtilis is most probably greater than 1271 kb.

The plasmid DNA incorporated in the scaffolds showed a burst release in the first week and a sustained release for the other 2 weeks.

The results showed that the DNA incorporated LBL film was fabricated successfully and the amount of ssDNA and BSA in the film could be tailored simply by controlling the number of the bilayers.

In Rubin's lab, postdoctoral fellow James Noonan first created a library of Neandertal DNA incorporated into live bacteria that reproduce the DNA, creating an endless supply.

Recent studies relating to direct effects from DNA incorporated 125I-molecules in plasmids have shown: If 125I has a critical distance to the DNA helix, the DSB generation switches from a direct to an indirect mechanism [16].

Both contigs referred to above as "organel.nuc" actually correspond to chloroplast tRNA and thus add to the probable contaminants resulting from plant DNA incorporated in our libraries.

Both positioning investigations established that G/C-rich sequences predominate at the center of an archaeal nucleosome but a 10 bp periodicity of AA/AT/TA/TT dinucleotides was not detected in the DNA incorporated into nucleosomes in Hlf.