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On the other hand, incubation at an elevated temperature after proteinase K digestion partially removes formalin cross-linking of the DNA, improving yield as well as DNA performance in assays.
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"These ideas are going to gain momentum as our understanding of DNA improves, and they can raise real ethical dilemmas," Woollard told the Guardian.
Detection of other methylated markers in stool DNA improved sensitivity with little effect on specificity.
Dilution of extracted DNA improved sensitivity only for badger faeces (Table 2, Fig. 1).
The demonstration that high levels of input genomic DNA improve robustness of genotyping results is paralleled by PCR results.
It has been demonstrated that DNA-AuNPs that were formed with double-stranded DNA improved the rate of target hybridization and DNA-mediated AuNP assembly.
To determine whether Dam methylated DNA improves transformation efficiency, unmethylated plasmid DNA was Dam methylated in vitro and transformed into C. thermocellum.
Such information will facilitate the selection of the most effective or desirable DNA construct for improving DNA vaccine potency.
But he says the ability to synthesize DNA is improving rapidly.
Thymine in the genetic code might cope for this as it was shown that thymine protected DNA and improved the efficiency of DNA replication [ 22].
Our multiple displacement amplification protocol employs a second strand-displacing DNA polymerase, improved buffers, improved reaction conditions and DNA free reagents.
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