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DNA extract was diluted 100-fold and directly used for real-time quantitative PCR Rotor-Gene QQiagengen).
Further, quantity and quality of DNA extract was influenced by the extraction method.
Both water controls and an extraction control consisting of a mammoth DNA extract were negative for cave bear-specific products.
The extraction was performed as described previously [ 49], yielding 70 μl of DNA extract.
In particular, HRM detected H. niger DNA extract in A. complanatus DNA extract at concentrations as low as 1%.
The DNA extract was used as a template for PCR amplification (Omar et al. 2014).
A final 100 μl DNA extract was eluted and stored at − 20 °C until molecular evaluation.
A series of PCR-based analyses was then used to assess DNA extract quality and effect on microbial community profiles.
Template was 1 µL of the DNA extract.
MCPyV promoter sequence was amplified from DNA extract of patient P18 skin sample.
All aurochs DNA extract aliquots yielded successful genomic libraries for Illumina GA sequencing.
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