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An injection of the engineered DNA boosted the efficacy of standard TB drugs: Six of 10 mice that got only drugs showed signs of reactivated infections while none of those receiving the combination treatment did, the researchers report in the 24 March issue of Gene Therapy.
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Another group immunised mice against mSTEAP using several vaccination protocols including a gene gun mSTEAP prime/ s.c. mSTEAP-Virus Replicon Particle (VRP) boost; gene gun mSTEAP prime/ s.c. mSTEAP DNA boost and mSTEAP-VRP prime and boost [56].
The pLAMP/gag-transfected dendritic cells prime and naked DNA boost immunization protocol also resulted in an increased apparent avidity of peptide in the ELISpot assay.
The 'rBCG prime - DNA boost' regimen employed in this study provides multiple advantages and possibilities in terms of its clinical relevance.
Hence, vaccination with 'rBCG prime - DNA boost' regimen based on α-crystallin is likely to reduce the incidence of latent and reactivation TB.
With a DNA boost at four weeks and a second readout of anti-Gag antibody titers measured at six weeks, the gap continues to widen between the MO and CO vaccines to 6-fold.
We show that "rBCG prime - DNA boost" strategy (R/D) confers a markedly superior protection along with reduced pathology in comparison to BCG vaccination in guinea pigs (565 fold and 45 fold reduced CFU in lungs and spleen, respectively, in comparison to BCG vaccination).
Lambracht-Washington et al. demonstrated a novel strategy of using peptide prime plus DNA boost immunization regimens [ 30].
While survival was significantly increased with all mSTEAP vaccination protocols, the most significant effect was seen in mice vaccinated with mSTEAP DNA and boosted with mSTEAP-VRP.
Group two was primed with plasmid DNA and boosted with VLP as described above.
We have previously described a four antigen malaria vaccine consisting of DNA plasmids boosted by recombinant poxviruses which protects a high percentage of rhesus monkeys against Plasmodium knowlesi (Pk) malaria.
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