Exact(4)
In this study, we developed a new multiplex PCR method coupled to DNA array hybridization, which can simultaneously detect all three human herpesviruses in one single cell sample.
As assessed by PCR and DNA array hybridization, clonal complexes were characterized by specific gene content.
Generally, the DNA array hybridization results were in accordance with housekeeping genes-based phylogeny and genotypic (ST) classification.
Recently, Han et al. used ITP to improve sensitivity and speed of DNA array hybridization, detecting twenty targets down to 100 fM in 30 min, also with <1% capture efficiency.
Similar(56)
RNA was collected from five independent cell platings and used for five separate DNA array hybridizations.
In contrast, DNA array hybridizations measure nucleotide conservation and are only capable of detecting highly conserved DNA sequences.
Here we compare the genomes of 26 L. monocytogenes strains representing the three lineages based on both in silico comparative genomic analysis and high-density, pan-genomic DNA array hybridizations.
These authors identified 101 dehydration-inducible transcripts by repetitive rounds of cDNA subtraction, differential DNA-array hybridization, and Northern-blot analysis.
Generally, addition of COT-1 DNA during array hybridization reduced the proportion of repetitive elements present in the captured DNA by 33%, thereby increasing the percentage of reads from non-repetitive genomic elements, and improving enrichment for the target sequence ∼14-fold ∼14-fold
Bisulfite modification of DNA and array hybridization was carried out by Cambridge Genomics Services.
Processing of genomic DNA and array hybridization were performed in accordance with the manufacturer's standard recommendations.
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