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(Culture volume was 1 ml per well containing 3×106 cells).
The spheroplast containing pellet was re-suspended in distilled H20 at one-tenth the volume of OD normalised culture volume.
Culture volume ranged from 500 to 1300 m3 for each land-based tank.
For RNA extraction, a culture volume (100 ml) was immediately mixed with an equal volume of RNAlater® (Ambion, USA).
Mature trophozoite-infected and schizont-infected RBCs were eluted with ice-cold PBS at one-fifth of the culture volume.
After nine months a FEP bag contained 8 ml of the 11 ml starting culture volume of liquid CeMM.
Final data was normalized with the respect of cell culture volume which results in a copy number of RNA and DNA per unit of culture volume.
Finally, the maximal speed of temperature changes is limited by equipment capabilities and the culture volume.
Subsequently, serial migration-dilution experiments were performed in well-mixed batch culture with a culture volume of 200 μL.
Samples were taken immediately (0 h) and after 8, 24 and 48 h, only causing a reduction of culture volume of less than 10% in total.
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