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Genome-predicted protein sequences were validated to correspond to transcripts by the identification of corresponding expressed sequence tags (ESTs) and/or cDNA sequences within transcriptome datasets.
Subsequently, HqL07 and HqL09 were expressed in vitro and the molecular weights of the corresponding expressed products were 60 and 70 kDa, respectively.
Genes selected from the rice and sorghum genomic sequences were used in BLASTN searches to find corresponding expressed sequence tags from other grass species.
To this end, SH-SY5Y cells were co-transfected with pDsRed2-mito and GFP-LC3 plasmids, and analyzed the localization of the corresponding expressed proteins by confocal microscopy.
In our study, 3952 primer pairs flanking the corresponding expressed sequences were designed based on cold-responsive transcripts of Elaeis guineensis.
Version 1.0 of the P. fucata draft genome contains 23 257 complete gene models, 70% of which are supported by the corresponding expressed sequence tags.
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Analysis of expression data confirmed that 36 of this modules (85%) corresponded to expressed transcripts.
Another 12 unique sequences were present in the nucleotide database resulting in a total number of around 100 unique sequences, which correspond to expressed genes from N. viridescens.
In total, 4194 sequences (89% of the 4700 probes corresponding to P. parasitica sequences or sequences of unknown origin) gave a hybridization signal stronger than the background in at least one condition and were considered to correspond to expressed genes.
The template of subclass A (template.A) is defined as a vector containing nA+nB elements: first nA elements correspond over-expressed genes in subclass A (marker.A), and next nB elements correspond over-expressed genes in subclass B (marker.B).
Corresponding genes are expressed in L. major [ 16].
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