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Control mutant Or42b − larvae also showed slightly elevated attraction to 10−1 ethyl acetate, consistent with previous trends.
Control mutant Or42b − larvae matched prediction by showing strongly reduced attraction to 10−4 ethyl acetate compared to wild type (ANOVA, Tukey Kramer, p<0.0001) (Fig. 2B).
Control mutant Or42a − larvae had greatly reduced behavioral attraction to 10−1 ethyl acetate compared to wild type as expected (ANOVA, Tukey Kramer, p<0.0001) (Fig. 2A).
Control mutant strains were also constructed by combining either the Or42a − allele or the Or42b − allele with Or42aGAL4 and Or42bGAL4, which should not show rescue and should display the respective mutant phenotypes.
Since tumor penetrance is inherently variable, for well-controlled conditions and comparable results, all control, mutant, rescue and overexpression animals were grown and scored simultaneously under the same conditions.
Neutral charge substitutions (K139A and K207A) retained greater tolerance and stability followed by negative charge substitutions (K139D and K207D) compared to control mutant K139R and wild enzyme.
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LS1, LS3, and the control mutants (Figure 1) were identified when grown in the greenhouse.
No difference was found between control and mutant females (Table 4, Figure 6A) or control and mutant males (Table 4).
Compared to the control, the mutant exhibited reduced resistance to the rice blast pathogen CH45.
For negative control, the mutant uncB (R169A) mRNA template was used.
It is crucial to control the mutant expression and not touch the normal huntingtin protein.
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